AccuraCode® TCR – High-Throughput TCR Sequencing
The AccuraCode® TCR Library Construction Kit enables efficient, accurate, and scalable exploration of T-cell repertoires. It simultaneously profiles TCRs from multiple samples and facilitates comprehensive analysis of T-cell clonotypes, gene usage, V(D)J recombination patterns, and diversity.
Kit Highlights
AccuraCode TCR kit detects rare T-cells accounting for as low as 1% of the total population
For this purpose CCRF-CEM cells were used, a cell line derived from an acute lymphoblastic leukemia (ALL) patient and known to consist of one clonotype only T-cell lymphoblasts. For the sensitivity testing, PBMCs were spiked with varying fractions of CCRF-CEM cells.
(A) Schematic overview of the experimental setup and workflow. PBMCs and CCRF-CEM cells were mixed at different proportions. Lysis was performed on plate prior to library generation. (B) Tested cell compositions. (C) AccuraCode TCR accurately detects the proportion of CCRF cells from the mixture at 1-20% CCRF frequency.
Streamlined and condensed plate-based workflow: from sample to library within one workday
Applications Areas
Technical Specifications
