Millions of DNA fragments are simultaneously sequenced, generating massive datasets, which can be assembled into a complete genome read, allowing for comprehensive genome analyses. Let your research shine with the future of genomic analysis at Azenta.
Whole Exome Sequencing and Targeted Sequencing
With new technologies from Agilent, we are able to bring to you the highest quality of sequencing. Targeted sequencing employs Next-Generation Sequencing to selectively analyze specific genes or genomic regions for swift identification of genetic mutations. This focused approach, exemplified by Whole Exome Sequencing, is cost-effective, making it valuable for exploring mutations in coding regions associated with diseases.
Whole Genome Sequencing
Whole genome sequencing (WGS) offers unparalleled access to genomic data, accelerating advancements in human healthcare, oncology, biomarker discovery, agriculture, and metagenomics, fostering breakthroughs across diverse fields.
1. Short-Read Human WGS: Illumina® NovaSeq™ or HiSeq®
2. Short-Read Non-Human WGS: Illumina® MiSeq®, HiSeq®, or NovaSeq™
3. Long-Read WGS: PacBio® Sequel®
4. Low-Pass Whole Genome Sequencing
16s/ITS/18s/Archaea & Shotgun Metagenomic Sequencing
Metagenomic sequencing, employing targeted assays like 16S and ITS2, as well as shotgun sequencing, utilizes next-generation sequencing (NGS) to swiftly analyze microbial community diversity. This approach reveals insights into the roles of individual species within these communities.
RNA sequencing (RNA-Seq) is a potent tool for comprehensive transcriptome analysis, providing qualitative and quantitative insights into gene expression. Single-Cell RNA-Seq, such as Azenta's optimized workflows, delves into single-cell transcriptome heterogeneity, revealing nuanced cellular differences hidden in bulk RNA sequencing.
Azenta Life Sciences provides advanced epigenomic sequencing solutions, including Methylation Sequencing for detecting 5-methylcytosine and PacBio® Methylation Sequencing for diverse DNA modifications. Their offerings extend to ATAC-Seq, ChIP-Seq, and Single-Cell ATAC-Seq, facilitating in-depth exploration of chromatin accessibility, protein-DNA interactions, and single-cell chromatin structure.
A more inexpensive way to sequence without the need for a reference genome is here. ddRADseq is a variation on the RAD sequencing protocol for SNP discovery and genotyping. It uses two restriction enzymes instead of fragment shearing for a more accurate size selection. Combinatorial indexing is also possible. Several variants of RAD have been developed, and multiple software packages are available for data analysis.
In this method, genomic DNA is digested with two restriction enzymes, ligated with adaptors, and size-selected. P2 primers are then ligated and the fragments amplified.
Plasmid-EZ offers complete, rapid, and cost-effective whole plasmid sequencing. Unlike traditional methods, it analyzes the entire plasmid, guaranteeing full verification and faster results.