CLindex®
– Multiplexing Made Easy
The CLindex® Sample Multiplexing Kit allows multiplexing of up to 16 libraries for single cell sequencing through click-chemistry-based sample tagging.
Kit Highlights
CLindex® Technology
The CLindex® multiplexing technology employs click-chemistry for efficient, unbiased sample tagging. A chemical group with a high affinity to cell surface proteins and a sample indexing oligo binding to that chemical group label the cells of each sample. A major advantage of the click-chemistry-based labeling compared to antibody-based labeling is that the cell surface proteins recognized by click chemistry are abundant and found across different species.
Streamlined Workflow
High Labeling Efficiency
Four cell lines (NB4, THP-1, U937, and CCRF) were labeled with CLindex®, pooled equally, and processed on the SCOPE-chip®. Cell clustering confirmed unbiased CLindex® sample indexing, maintaining tag proportions. Labeling efficiency reached 97%, with only 3% multiplets or undetermined. CLindex® tagging doesn’t affect capturing, sequencing, mapping, or overall gene expression compared to non-labeled pooled cells.
Unbiased Labeling Across Different Species
CLindex® efficiently labels conserved cell surface proteins. In a study with 16 samples (8 mouse testis, 8 human CCRF cells), CLindex®-tagged samples were pooled, processed using HD SCOPE-chip®, capturing and sequencing over 40,000 cells. Pooled samples displayed equal representation with a low multiplet rate.
Technical Specifications
